Some bacteria have another method of transferring dna and producing recombinants that does not require conjugation. In this part of the lab, you will introduce a gene for resistance to the antibiotic ampicillin into a bacterial strain that is killed by ampicillin. The art of bacterial transformation introduction purpose. Americas lab report looks at a range of questions about how laboratory. Reagents, protocols, and background information for the classic pglo bacterial transformation lab plus four.
Observe the results you obtained from the transformation lab under normal room lighting. Information for teacherssafety instructions although the e. Ap biology 20062007 science academy writing a lab report for ap biology each semester, you are required to turn in a full lab writeup. How to write a biology lab report for school examples. The crash course is based on the latest changes to the ap biology course curriculum and exam. Ap biology bacterial transformation lab by kenneth. Transformation lab a plasmid discovery labratory 6, ap biology abstract. How to write a biology lab report for school if youre taking up biology classes, chances are you are tasked to do lab experiments and of course, lab reports. However, with this specific lab especially i found the writing of the lab report was just as educational if not more than the actual. Note that any related adjustments to 2020 ap exams, such as length or content covered, may not be reflected on all. Education center k12 lessons and laboratories classroom activities in plant biotechnology. Coli lab write up what students are saying as a current student on this bumpy collegiate pathway, i stumbled upon course hero, where i can find study resources for nearly all my courses, get online help from tutors 247, and even share. Ap biology 20202021 statement of understanding by signing this contract, the parent and the student acknowledge that they have read the preceding documentation for mrs.
Choose from our kits, follow a college board lab, or design your own with our wide variety of equipment and supplies. Ap biology syllabus 20202021 instructors information. Product details transformation involves the uptake of extracellular dna by another cell, resulting in a newly acquired genetic trait. Mcgrawhill virtual labs the mcgraw hill virtual lab is great just print off the post lab questions and tables and have students complete the lab ap environmental weblabs uca labs with good descriptions and great standard and simulation labs. This is in addition to the regular lab writeup analysis and assessment questions that you have been used to completing for each lab. In the transformation lab, we discovered the process of bacterial genetic transformation and how to calculate transformation efficiency. Ap biology transformation lab report free download as text file. Prepare your students for medical and lab tech careers with carolina s wide range of equipment, kits and models. Transformation is the most likely mechanism by which dna can be transmitted from a eukaryotic organism to bacteria. We have kits for new and traditional ap biology labs. In a molecular biology laboratory, a student obtained competent e. Bacterial transformation advanced student laboratory kit.
What i learned from this lab impressions of the pglo lab. Coli to include a plasmid containing the lux operon, making the bacteria luminescent. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Paul andersen explains the two major portions of the molecular biology lab in ap biology. Introduction abstract our alternate hypothesis for this experiment will be that the pglo dna will incorporate in the e. Summarize the overall idea of the procedures in a few steps. Completing the pglo experiment was really useful for learning about the processes of bacterial transformation and how to properly follow the lab procedure. This updated ap biology lab manual teacher edition includes revised big ideas. During the first semester, you may write up either lab 5 or lab 4. Depending on the shapes and structures of the enzymes, the function could change drastically. Some of the recommended labs may be too expensive or too time consuming for your class. Transformation lab report at end of class period fern minimarsh observations read. After 30 minutes, remove the tubes from the water bath and place on lab bench. Classic bacterial transformation lab using pglo plasmid from biorad.
If your instructor requires a separate title page, include the title followed by the names of the project participants, class title, date, and instructors name. Protocol is a mixture of two protocols from following lab books. Antibiotic use of antibiotic resistancesensitivity genes detailed description of antibiotic resistance lab procedure gel electrophoresis isolate plasmid using electrophoresis. Genetic transformation occurs when a host organism takes in foreign dna and expresses the foreign gene. They span the globe in their topics and their techniques. Our easytoread format gives students a crash course in biology. Ap biologyinvestigation 8bacterial transformation lab. Wingerdens ap biology class and that they understand and agree to the commitment necessary to be successful in this course. Big genetics and information transfer 3 ap central. Transformation was discovered in streptococcus pneumoniae in 1928 by frederick griffith.
The lbamp pglo plate will have no growth, the lb pglo plate will have a lawn of growth meaning colonies covering. Enzyme activityoxygen sensor ap biology investigation. Transforming li strains with green fluorescent protein. Natural genetic transformation is the active uptake of free dna by bacterial cells and the heritable incorporation of its genetic information. Transpiration lab report professional academic writers. As a member, youll also get unlimited access to over 79,000 lessons in math, english, science, history, and more. This means that they increase the speed of chemical reactions in biological systems by lowering the activation energy needed to start reacting. The ap college board lists labs for its recommended curriculum, however, teachers are not limited to only using their versions of the lab. Therefore, the activity of the enzyme varies according to ph. Bacterial gene transfer by natural genetic transformation. Since 1989, there have been 12 laboratorieslovingly nicknamed the dirty dozenthat are suggested curricula for ap biology classes. The conversion of one genotype into another by the introduction of exogenous dna that is, bits of dna from an external source is termed transformation. Ap biology ap biology formal lab report guidelines prepare a written report of your experiment which includes the section titles listed below.
Transformation lab report an experiment to incorporate lux operon containing plasmids into e. Ap biology weblabs this site has a virtual lab on each of the dirty dozen ap biology labs. Answer the lab packet questions as you proceed through the virtual lab. Please sign, remove this page from the packet, and return it to mrs. Bacterial transformation an introduction to genetic. See the course schedule or browse the youtube playlist.
These section titles should be used to label each section of your report. Biology lab experiments range from dissecting a certain creature, exploring different organisms that are not visible to the naked eye, making products out of natural products, or just. It applies knowledge gained in several of the other labs, especially lab 4 on photosynthesis and lab 5 on cellular respiration. In both cases, youll want to supplement these notes with information from an ap biology textbook or. Prepare your students for medical and lab tech careers with carolina s wide range of. Select lab 6 molecular biology part i transformation in bacteria 3. In the transformation lab designed by the carolina biological supply co. In this experiment, the bacterial transformants acquire antibiotic resistance and a blue color, following the uptake of a plasmid carrying genes with. View lab report 3867838apbiologytransformationlabreport from microbiolo at national university of singapore. Lab report rubric print, attach and selfgrade for every formal lab report that you hand in lab report procedure and grading lab notebook grading rubric print and. Coli bacterial cells through the transformation process carolina biological supply co.
Editorial inquiries concerning this book should be directed to the college board. Bacterial transformation transformations student sheet. Ap bio bacterial transformation flashcards quizlet. Reas ap biology crash course is the first book of its kind for the lastminute studier or any ap student who wants a quick refresher on the course. The notes in this article can be used to study for ap biology tests throughout the year that deal with smaller portions of the curriculum, or they can be referenced in your studying for the final ap exam. Ap biology investigative labs ap central the college board. May 2016 march 2016 january 2016 december 2015 november 2015 october 2015. This web page was produced as an assignment for an ap biology course at montgomery high school. Plus, get practice tests, quizzes, and personalized coaching to help you succeed.
In the bacterial transformation advanced inquiry lab kit for ap biology, study biotechnology, which raises a lot of questions about the ethics of modifying. Ap biology transformation lab report transformation genetics. Ravesiweinstein science coordinator attleboro high school 100 rathbun willard dr. When lab is complete, collect all petri dishes, open, and immerse in a 10%. He starts by discussing the process of transformation. Ap biologyinvestigation 8bacterial transformation lab walkthrough halim sakarya. Describe plasmid uptake and how transformation is determined 6 points maximum.
Ap biology lab manual for teachers revised edition by college board author isbn. Open the tubes and using a 100 l micropipette with a sterile tip, transfer 250 l of the ice cold transformation solution cacl. Since the famous discovery of transformation in streptococcus pneumoniae by griffith in 1928 and the demonstration of dna as the transforming principle by avery and coworkers in 1944, cellular processes. The answers will vary but should be in the range of 103 104 transformantsg dna. Ap biology lab manual for teachers supplement technology integration use of gas pressure probeware provides a different means of running lab 2 a more rapidly, b without the necessity of using caustic sulfuric acid or messy permanganate stain and c without timing problems involved in stopping the reaction at specific points. Presentation on the alu insertion lab performed in the cold springs harbor lab.